Characterization of a bicistronic knock-in reporter mouse model for investigating the role of CABLES2 <i>in vivo</i>
نویسندگان
چکیده
Two members of the CDK5 and ABL enzyme substrate (CABLES) family, CABLES1 CABLES2, share a highly homologous C-terminus. They interact associate with cyclin-dependent kinase 3 (CDK3), CDK5, c-ABL. mediates tumor suppression, regulates cell proliferation, prevents protein degradation. Although Cables2 is ubiquitously expressed in adult mouse tissues at RNA level, role CABLES2 vivo remains unknown. Here, we generated bicistronic knock-in reporter mice that tagged 3×FLAG 2A-mediated fluorescent tdTomato. Cables2-3×FLAG-2A-tdTomato (Cables2Tom) confirmed expression various tissues. Interestingly, high intensity tdTomato fluorescence was observed brain, testis ovary, especially corpus luteum. Furthermore, immunoprecipitation analysis using brain Cables2Tom/Tom revealed interaction CDK5. Collectively, our new model will enable comprehensive function.
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ژورنال
عنوان ژورنال: Jikken Dobutsu
سال: 2021
ISSN: ['0007-5124', '1884-4162', '1884-4170', '1881-7122', '1341-1357']
DOI: https://doi.org/10.1538/expanim.20-0063